利用3' poly-A tailing及5' ligation of an adaptor sequence,來延長mature miRNA以反轉所有的mature miRNA,再搭配 TaqMan™ Advanced miRNA Assays 進行所有mature miRNA的偵測。
Kit內含:
• 10X PolyA Buffer
• ATP, 10mM
• Poly A Enzyme, 5 U/μL
• 5X Ligase Buffer
• RNA Ligase, 10 U/μL
• 50% PEG 8000
• 25X Ligation Adaptor
• 10X RT Enzyme Mix
• 20X Universal RT Primer
• dNTP Mix, 100mM
• 20X miR-Amp Primer Mix
• 5X RT Buffer
• 2X miR-Amp Master Mix