IMAP is a technology based on the specific, covalent-coordinate, high-affinity interaction of trivalent metal containing nanoparticles with phosphogroups. These phosphogroups can be free, linked to serines, threonines or tyrosines, or other molecules which make IMAP a generic platform to assess kinase, phosphatase and phosphodiesterase activity. This basic principle has been used in the IMAP Binding System using both fluorescence polarization and TR-FRET (as a read-out).