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GPCR Assays
Transfluor® technology (Cell-based fluorescence assay for GPCR ligands)
 

Transfluor is a cell-based fluorescence assay used to screen for G-protein-coupled receptors (GPCRs) ligands and other potential drugs that regulate GPCRs. This patented technology provides a powerful functional assay for detecting a compound's activity against known and/or orphan GPCR targets. Transfluor is optimized for HCS; secondary screening and lead optimization.

Product Instruction:

Works with all GPCRs (known, orphan, Gi, Gs, Go, Gq)

Single read-out for all GPCRs (Gi, Gs, Go, Gq)
No GPCR labeling or tagging
Requires no prior knowledge of interacting G-protein
Eliminates the need for multiple GPCR assays
Ideal for high content screening (HCS) analysis
Validated in over 100 GPCRs (from all classes)
Compatible with most commercially available HCS instruments
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FLIPR® Calcium Assay Kit
 

The Calcium Flux Assay Kit from Molecular Devices Corporation was developed and optimized for FLIPR to provide a fast, simple and reliable fluorescence based assay for detecting changes in intracellular calcium.

Product Instruction:

Stronger signals
Higher assay precision
Improved physiological data
No need to wash cells
Higher sample throughput in fewer hands-on steps
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FLIPR® Calcium 3 Assay Kit (Superior Detection for Diversified Targets)
 

The FLIPR Calcium 3 Assay Kit provides a universal method for detecting changes in intracellular calcium concentration in a simple and reliable homogeneous assay format.

Product Instruction:

No wash steps

 Higher signal intensity Compatible with more targets
Adherent and non-adherent cell types Improved data quality    
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FLIPR® Calcium 4 Assay Kit
       (A next-generation No-Wash, homogeneous, calcium mobilization assay Designed for the most challenging
       and difficult GPCR targets)
 

Product Instruction:

Lower background and increased signal intensity

 Significant reduction of ligand or target interference Compatible with an extensive range of targets and cells
Minimal addition artifact Strong signal with or without media removal    
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CatchPoint® Cyclic AMP Assay Kit (Reagents for cyclic-AMP Detection)
 

The CatchPoint cyclic-AMP Assay Kit measures cAMP levels and adenylate cyclase activity via a competitive immunoassay format. The assay's high affinity reagents are optimized for sensitivity and precision in applications where cAMP levels are low.


Product Instruction:

Sensitive detection limit: 6 fmol cAMP/well

 Robust format, resists interference from compounds Rapid signal development (< 10 min)
No termination step, flexible read time Compatible with high throughput screening (HTS) Detection limit: 24 fmol/well for 96 well format
Detection limit: 12 fmol/well for 384 well format        
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CatchPoint® Cyclic GMP Assay Kit
 

The CatchPoint® cyclic-GMP fluorescent assay kit measures cGMP levels and guanylate cyclase activity via a competitive immunoassay format. The assay's high affinity reagents are optimized for convenience with high sensitivity and precision. This is particularly important in applications where cGMP levels are low.

Product Instruction:
More sensitive and less susceptible to interferences than homogeneous assays Requires only a single wash step Rapid signal development (< 10 min)
Flexible read time, compatible with high throughput screening (HTS) Convenient, no termination step needed Detection limit: 24 fmol/well for 96-well format
Detection limit: 12 fmol/well for 384-well format
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LiveWare®cell lines and plasmid vectors for cAMP-coupled GPCR assays.
 

LiveWare reagents have been successfully used on the FLIPR system, in conjunction with non-G q -coupled members of the following receptor families: ：
α-adrenoceptors Chemokines Glutamate
Serotonin β-adrenoceptors Dopamine
Opioid Vasopressin    

 
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