 Best-In-Class Microplate Solutions |
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| Assays Type |
Absorbance (UV/VIS) Microplate Readers |
Luminescence Microplate Readers |
| SpectraMax Plus 384 |
SpectraMax 190 |
SpectraMax 340PC 384 |
VersaMax |
VMax |
EMax |
SpectraMax L |
| ELISA/ immunoassays |
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Protein Assays
‧ UV (280)
‧ Bradford, BCA, Lowry
‧ NanoOrange™ ,
CBQCA |
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Nucleic acid assays
‧ UV(260/280)
‧ PicoGreen™ ,
OliGreen™
RiboGreen™
‧ DNAQuant™ |
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Genomics
‧ SNP
‧ Molecular Beacons |
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Reporter genes
‧ Alk. phos , β-
galactosidase
‧ β -lactamase
‧ luciferase dual
‧ luciferase
‧ GFP |
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Cell viability/proliferation/death
‧ MTT, XTT, crystal violet
‧ 340-LDH
‧ alamar blue
‧ calcein AM,
CyQuant ™ GR
‧ SYTOX Green,
ethidium homodimer |
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Chemical compound
‧ ATP
‧ Superoxides |
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Cell signaling
‧ selected aequorin Ca 2+
‧ Ca mobilization
‧ Membrane potential |
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Enzyme Assays
‧ Protease
‧ NADH
‧ GST
‧ Phosphotases/Kinases |
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| Microbial growth |
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PATHCHECK
‧ pipettor calibration(to4μl)
‧ automatic correction to1cm |
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| Spectral scanning |
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| Fluorescence spectrum |
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| Cuvettes & Test tubes |
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ADME
‧ Cell adhesion |
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Endotoxin
‧ Turbidimetric
‧ Colorimetric |
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Binding Assays
‧ Fluorescence polarization
‧ TR-FRET |
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Multimode Readers Comparison Chart |
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SpectraMax M5/M5e |
SpectraMax M2 |
SpectraMax M2e |
FlexStation 3 |
| Primary detection modes |
FI,FP,UV/VIS Abs,TRF,Lum |
FI,UV/VIS Abs |
FI,UV/VIS Abs |
FI,FP,UV/VIS Abs,TRF,Lum |
| Secondary detection modes |
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TRF,Lum |
TRF,Lum |
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| wavelength selection |
Tunable , monochromator |
Tunable , monochromator |
Tunable , monochromator |
Tunable , monochromator |
| Wavelength range (Abs) |
200-1000 nm |
200-1000 nm |
200-1000 nm |
200-1000 nm |
| Wavelength range (FI) |
250-850 nm |
250/360-850 nm |
250-850 nm |
250-850 nm |
| Bandwidth |
Abs: 4 nm FI: Ex 9 nm, Em 15 nm |
Abs: 4 nm FI: 9 nm |
Abs: 4 nm FI: 9 nm |
Abs: 4 nm FI: Ex 9 nm, Em 15 nm |
| Cuvette |
Abs,FI,Lum |
Abs,FI |
Abs,FI |
No |
| Microplate formats |
6-384 |
6-384 |
6-384 |
6-384 |
| Cuvette s & Test tubes |
Yes |
Yes |
Yes |
No |
| Integrated multi-channel pipettor |
No |
No |
No |
8-channel/16-channel Pipettor |
| Endpoint read |
Yes |
Yes |
Yes |
Yes |
| Kinetic read |
Yes |
Yes |
Yes |
Yes |
| Fast Kinetic |
No |
No |
No |
Yes |
| Well scanning |
Yes |
Yes |
Yes |
Yes |
| Wavelength-selectable luminescence |
Yes |
No |
No |
Yes |
| Spectral scanning |
Yes |
Yes |
Yes |
Yes |
| PathCheck |
Yes |
Yes |
Yes |
Yes |
| Temperature control |
Ambient + 2 ℃ to 60 ℃ |
Ambient + 4 ℃ to 45 ℃ |
Ambient + 4 ℃ to 45 ℃ |
Ambient + 2 ℃ to 60 ℃ |
| Mixing/Shaking |
Yes |
Yes |
Yes |
Yes |
| Plate handing options |
SynchroMax ET |
SynchroMax ET |
SynchroMax ET |
SynchroMax ET
or integrated stacker |
| Bottom-reading |
FI,TRF,Lum |
No |
FI,TRF,Lum |
FI,TRF,Lum |
| FDA 21 CFR Part 11 Compliance |
Yes |
Yes |
Yes |
Yes |
| Integrated stacker |
Yes |
Yes |
Yes |
Yes |
| HTRF certified |
Yes (M5 e only) |
No |
No |
Yes |
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Absorbance Readers Comparison Chart |
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SpectraMax
Plus 384 |
SpectraMax190 |
SpectraMax 340PC 384 |
VersaMax |
VMax |
EMax |
| VIS absorbance detection |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
| UV absorbance detection |
Yes |
Yes |
No |
No |
No |
No |
| Wavelength selection |
Yes |
Yes |
Yes |
Yes |
No |
No |
| Wavelength range (nm) |
190-1000 |
190-850 |
340-850 |
340-850 |
400-750 |
400-750 |
| Bandwidth (nm) |
2 |
2 |
2 |
2 |
10 |
10 |
| Cuvette s & Test tubes |
Yes |
No |
No |
No |
No |
No |
| Microplate formats |
96/384 |
96 |
96/384 |
96 |
96 |
96 |
| Endpoint read |
Yes |
Yes |
Yes |
Yes |
Yes |
Yes |
| Kinetic read |
Yes |
Yes |
Yes |
Yes |
Yes |
No |
| Spectral scanning |
Yes |
Yes |
Yes |
No |
No |
No |
| PathCheck |
Yes |
Yes |
Yes |
No |
No |
No |
| Temperature control |
Ambient + 4 ℃ to 45 ℃ |
Ambient + 4 ℃ to 45 ℃ |
Ambient + 4 ℃ to
45 ℃ |
Ambient + 4 ℃ to 45 ℃ |
No |
No |
| Mixing/Shaking |
Yes |
Yes |
Yes |
Yes |
Yes |
No |
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Fluorescence Readers Comparison Chart |
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Gemini XPS |
Gemini EM |
FlexStation II 96/384 |
| Fluorescence |
Yes |
Yes |
Yes |
| Luminescence |
Secondary mode |
Secondary mode |
Secondary mode |
Time-resolved
fluorescence (TRF) |
Secondary mode |
Secondary mode |
Secondary mode |
| Wavelength selection |
Tunable,Monochromator |
Tunable,Monochromator |
Tunable,Monochromator |
Wavelength range
(nm) |
250-850 ex360-850 em |
250-850 ex360-850 em |
250-850 ex360-850 em |
| Bandwidth (nm) |
9/9 |
9/9 |
9/18 |
| Microplate formats |
6,12,24,48,96,384 |
6,12,24,48,96,384 |
6,12,24,48,96/384 |
| Endpoint read |
Yes |
Yes |
Yes |
| Kinetic read |
Yes |
Yes |
Yes |
| Spectral scanning |
Yes |
Yes |
Yes |
| Fast Kinetic read |
No |
No |
Yes |
| Temperature control |
Ambient + 4 ℃ to 45 ℃ |
Ambient + 4 ℃ to 45 ℃ |
Ambient + 4 ℃ to 45 ℃ |
| Mixing/Shaking |
Yes |
Yes |
Yes |
| Top - reading |
Yes |
Yes |
Yes |
| Bottom-reading |
No |
Yes |
Yes |
| Integrated multi-channel pipettor |
No |
No |
8-channel/16-channel Pipettor |
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Lurninescence Microplate Reader |
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‧ SpectraMax® L Microplate Reader |
Microplate Data Acquistion and Analysis Software |
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‧ SoftMax® Pro 5
‧ Validation Packages
‧ SpectraTest ™ |
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