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 Real-time PCR |
 StepOne |
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| Thermal Cycling System |
Peltier-based system |
| Block Format |
• 48-well block |
| Compatible Consumables |
• 48-well plates with optical flat caps |
• 48-well plates with optical adhesive
cover |
• 8-tube (0.1 mL) strips with optical flat
caps |
• Individual (0.1 mL) tubes with optical
flat caps |
| Supported Volumes |
10–30 μL |
| Sample Ramp Rate |
• Fast mode: +/- 2.2 °C/sec |
| • Standard mode: +/- 1.6°C/sec |
| Peak Block Ramp Rate |
4.6 °C/sec |
| Temperature Range |
4°C-100°C |
| Temperature Accuracy |
+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start. |
| Temperature Uniformity |
+/-0.50°C, 30 seconds after clock start. |
| Melt Curve Resolution |
As small as 0.1°C |
| Optical System |
LED, emission filters, and photodiode |
| Calibrated Dyes at Installation |
SYBR® Green I, FAM™, VIC®, JOE™, ROX™ dyes |
| Passive Reference Dyes |
ROX™ or any calibrated dye. Option exists to select no passive reference. |
| Data Collection |
Data collected in all 3 filters for all wells regardless of plate setup. |
| Plate setup may be altered after run completes. |
| Fast cycling: Less than 40 minutes |
| Quantitative PCR Run Time |
Standard cycling: Less than 2 hrs |
| Fast cycling: Less than 40 minutes |
| Touchscreen |
LCD/6.5 in. Full VGA (640x480)/32K colors |
| Instrument Dimensions |
• Width: 24.6 cm (9.7 in.) |
| • Depth: 42.7 cm (16.8 in.) |
| • Height: 51.2 cm (20.2 in.) |
| • Weight: 23.6 kg (52 lbs.) |
| Electrical |
US/Canada: |
| • Voltage: 120 VAC ± 10% |
| • Frequency: 50/60 Hz ± 1% |
| • Nominal Current Draw: 8A |
| • Power: 950 W |
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StepOnePlus |
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| VeriFlex™ Block |
25°C (5°C zone-to-zone) |
| Thermal Cycling System |
Peltier-based system |
| Block Format |
96-well block |
| Compatible Consumables |
• 96-well (0.1 mL) plates with optical
flat caps |
• 96-well (0.1 mL) plates with optical
adhesive cover |
• 8-tube (0.1 mL) strips with optical flat
caps |
| • Individual (0.1 mL) tubes with optical flat caps |
| Supported Volumes |
10–30 μL |
| Sample Ramp Rate |
• Fast mode: +/- 2.2 °C/sec |
| • Standard mode: +/- 1.6°C/sec |
| Peak Block Ramp Rate |
4.6 °C/sec |
| Temperature Range |
4°C-100°C |
| Temperature Accuracy |
+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start. |
| Temperature Uniformity |
+/-0.50°C, 30 seconds after clock start. |
| Melt Curve Resolution |
As small as 0.1°C |
| Optical System |
Blue LED excitation source, four-emission filters, and photodiode |
| Calibrated Dyes at Installation |
FAM™, SYBR® Green I, VIC®, JOE™, NED, TAMRA, ROX™ dyes |
| Passive Reference Dyes |
ROX™ or any calibrated dye. Option exists to select no passive reference. |
| Data Collection |
Data collected in all 3 filters for all wells regardless of plate setup. |
| Quantitative PCR Run Time |
Standard cycling: Less than 2 hrs |
| Fast cycling: Less than 40 minutes |
| Touchscreen |
LCD/6.5 in. Full VGA (640x480)/32K colors |
| Instrument Dimensions |
• Width: 24.6 cm (9.7 in.) |
| • Depth: 51.2 cm (20.2 in.) |
| • Height: 51.2 cm (20.2 in.) |
| • Weight: 24 kg (53 lbs.) |
| Electrical |
US/Canada: |
| • Voltage: 120 VAC ± 10% |
| • Frequency: 50/60 Hz ± 1% |
| • Nominal Current Draw: 8A |
| • Power: 950 W |
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7300 |
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| Thermal Cycling System |
Peltier-based system |
| Block Format |
96-well block |
| Compatible Consumables |
96-well plates and 0.2 mL tubes |
| Supported Volumes |
20 – 100 μL |
| Sample Ramp Rate |
+/- 1.1°C/sec |
| Peak Block Ramp Rate |
1.6°C/sec |
| Temperature Range |
4°C-100°C |
| Temperature Accuracy |
+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start. |
| Temperature Uniformity |
+/-0.50°C, 30 seconds after clock start. |
| Optical System |
Tungsten-halogen lamp excitation source. |
| Single-excitation filter, four-emission filters, and CCD camera |
| Calibrated Dyes at Installation |
SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™ |
| Passive Reference Dyes |
ROX™ or any calibrated dye. Option exists to select no passive reference. |
| Additional Dyes Available |
Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the User's Manual. Purchase of additional filter sets is not necessary. |
| Data Collection |
Data collected in all 4 filters for all wells regardless of plate setup. |
| Plate setup may be altered after run completes. |
| Quantitative PCR Run Time |
Less than 2 hrs |
| Electrical |
US/Canada: |
| • Voltage: 120 VAC ± 10% |
| • Frequency: 50/60 Hz ± 1% |
| • Nominal Current Draw: 8A |
| • Power: 950 W |
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7500 |
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| Thermal Cycling System |
Peltier-based system |
| Block Format |
96-well block |
| Compatible Consumables |
96-well plates and 0.2 mL tubes |
| Supported Volumes |
20 – 100 μL |
| Sample Ramp Rate |
• Standard Mode: +/-1.6°C/sec |
• 9600 Emulation Mode: +0.8 and
-1.6°C/sec |
| Peak Block Ramp Rate |
2.5°C/sec |
| Temperature Range |
4°C-100°C |
| Temperature Accuracy |
+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start. |
| Temperature Uniformity |
+/-0.50°C, 30 seconds after clock start. |
| Optical System |
Tungsten-halogen lamp excitation source. |
| Five-excitation filters, five-emission filters and CCD camera |
| Calibrated Dyes at Installation |
SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™, Texas Red®, Cy3™, Cy5™ |
| Passive Reference Dyes |
ROX™ or any calibrated dye. Option exists to select no passive reference. |
| Additional Dyes Available |
Calibration for new dyes within the wavelength range is possible by following the custom dye calibration procedure in the User's Manual. Purchase of additional filter sets is not necessary. |
| Data Collection |
Collects data for all 5 filters for all wells regardless of plate setup. |
| Plate setup may be altered after run completes. |
| Quantitative PCR Run Time |
Less than 2 hrs |
| Electrical |
US/Canada: |
| • Voltage: 120 VAC ± 10% |
| • Frequency: 50/60 Hz ± 1% |
| • Nominal Current Draw: 8A |
| • Power: 950 W |
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7900HT |
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| Thermal Cycling System |
Peltier-based system |
| Block Format |
• 96-well block (Standard) |
| • 96-well block (Fast) |
| • 384-well block |
| • Low Density Array block |
| Compatible Consumables |
• 96-well plates (Standard) |
| • 96-well plates (Fast) |
| • 384-well plates |
| • Low Density Arrays |
| Supported Volumes |
• 96-well Standard: 25-100 μL |
| • 96-well Fast: 10-30 μL |
| • 384-well: 5-20 μL |
| Sample Ramp Rate |
• Fast Mode: Ave. +/- 3.0°C/sec |
| • Standard Mode: +/-1.6°C/sec |
| • 9600 Emulation Mode: +0.8 and -1.6°C/sec |
| Peak Block Ramp Rate |
• 96-well (Fast): 4.8°C/sec |
| • 96-well (Standard), 384-well, Low Density Array: |
| 2.0°C/sec |
| Temperature Range |
4°C-100°C |
| Temperature Accuracy |
+/-0.25°C (35°C to 95°C) of setpoint/display temperature, measured at 3 minutes after clock start. |
| Temperature Uniformity |
+/-0.50°C, 30 seconds after clock start. |
| Optical System |
Extended-life 488 nm argon-ion laser excitation source. |
| Excitation light distributed to all wells via a |
| dual-axis synchronous scanning head. |
| Fluorescence detection via spectrograph and CCD camera |
Calibrated Dyes at
Installation |
Low Density Array: FAM™, VIC™, ROX™ |
| All other blocks: SYBR® Green Dye I, FAM™, VIC™, JOE™, NED™, ROX™, TAMRA™, TET™ |
| Passive Reference Dyes |
ROX™ or any calibrated dye. Option exists to select no passive reference. |
| Data Collection |
Collects data for all detectable fluorescence within the spectrograph range. |
| Plate setup may be altered after run completes. |
| Quantitative PCR Run Time |
33 minutes (96–well in fast mode), 52 minutes (384-well in fast mode), |
| <2 hrs (96 and 384-wells in standard/9600 emulation mode and Low Density Array) |
| Electrical |
• Voltage: 200-240 VAC ± 10% |
| • Frequency: 50-60 Hz ± 1% |
| • Maximum Current Draw: 16A |
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